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. Further, the presence of intact rod-like actin filament in the pupal retinal degeneration mutant background allowed us to observe the fates of the rod spherules in living pupal retinas by immunofluorescence.

The F-actin in the spherules stained red ([Figure 2](#f2){ref-type=”fig”}), and the bright punctate F-actin staining co-localized with DIC, which showed a high level of background fluorescence in the cytoplasm. This staining was prominent in the region between the rod spherules, suggesting that it was associated with the remaining R1, R2, or R3 rod spherules in the mutant retina. This punctate F-actin was similar to the staining pattern of de novo polymerized F-actin seen after photobleaching ([@bib21]; [@bib2]) ([Figure 4](#fig4){ref-type=”fig”}, compare A and B). We also observed co-localization of actin staining with JNK, particularly in the region between rod spherules and between them and the outer nuclear layer. The F-actin staining was similar to that which is seen in pyknotic photoreceptors ([Figure 5](#fig5){ref-type=”fig”}, compare A and B). One interpretation of the punctate actin staining pattern seen in the *w^1118^* retina is that it represents a pool of active JNK sufficient to induce the death of rod photoreceptors ([@bib12]; [@bib19]; [@bib23
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