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\nChemosensation plays an important role in the discovery of medicinal compounds and is important for homeostasis and survival of the body. This study aimed to develop a new and effective in vitro model of human carotid body type I-like chemosensory receptor. The human carotid body type I-like chemosensory receptor was cloned from bovine carotid body (CCB-type) cDNA library and expressed in vitro. First, the CCB-type receptor was transiently expressed in HEK293T cells. Then, the potential effect of receptor stimulation on cell proliferation was assessed by cell counting kit-8 (CCK-8) cell proliferation assay and immunofluorescence. The recombinant receptor signal pathway was assessed by western blot and immunofluorescence. The cell surface expression of CCB-type receptor was further confirmed with electron microscopy. In vitro transfection of the receptor in HEK293T cells led to the high level of cell expression of the receptor, as revealed by immunofluorescence and western blot. Furthermore, the significant effect of receptor stimulation with ATP (10 nmol\/L) on cell proliferation was observed. The stimulation of the receptor
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