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Previous reports on the effects of TCDD on energy metabolism showed results that are difficult to reconcile with the reported occurrence of insulin resistance in humans. To clarify these contradictory results, we evaluated the effects of TCDD on the expression of genes involved in the regulation of glucose metabolism in the liver and muscle of mice neonatally exposed to TCDD. Female Swiss mice received s.c. injections of either corn oil (vehicle) or 0.01, 0.1, or 1 microg TCDD\/kg body weight every 24 h from postnatal day (PND) 1 to 14. On PND 15, mice were sacrificed for molecular analyses. TCDD induced dose-dependent decreases in body weight and feed intake on PND 10 and PND 14. TCDD also reduced insulin levels on PND 10. Our results show that TCDD altered the expression of genes involved in the control of glucose metabolism in the liver and muscle, which were more pronounced at the higher dose. 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